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KMID : 0880220170550080640
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Journal of Microbiology
2017 Volume.55 No. 8 p.640 ~ p.647
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Effect of amikacin on cell wall glycopeptidolipid synthesis in Mycobacterium abscessus
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Lee So-Young
Kim Hee-Youn
Kim Byoung-Jun
Kim Hong
Seok Seung-Hyeok
Kim Bum-Joon
Kook Yoon-Hoh
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Abstract
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Cultivation of the smooth colony Mycobacterium abscessus at the sub-minimum inhibitory concentration (MIC) of amikacin changed its growth pattern including its colony morphology (smooth to rough) and cell arrangement (dispersed to cord formation). In addition, reduced sliding motility and biofilm formation were observed. The amount of glycogpetidolipid (GPL) and mRNA expression of key genes involved in GPL synthesis were decreased in the amikacin-treated M. abscessus strain. An in vitro infection assay revealed that the amikacin-treated smooth M. abscessus strain induced more pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) than that of the smooth strain in murine macrophage cells. These results suggest that long-term exposure to a low concentration of amikacin causes a physical change in the cell wall which may increase its virulence.
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KEYWORD
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Mycobacterium abscessus, glycopeptidolipid (GPL), amikacin, biofilm
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